Estudio fitoquímico y evaluación de la actividad antioxidante de Esenbeckia litoralis Donn.Sm. (Loro grande) / Phytochemical study and evaluation of the antioxidant activity of Esenbeckia litoralis Donn. Sm. (loro)

Introducción: la especie Esenbeckia litoralis Donn.Sm. (Rutaceae) es también conocida como Loro o Loro grande, ha sido empleado en la medicina tradicional para el tratamiento de mordeduras de serpientes, dolor de garganta y lesiones ocasionadas por picaduras de insectos. Esta variedad de actividades son causadas por acción de los compuestos que la constituyen, como lignanos, terpenos, alcaloides, cumarinas y polifenoles que representan un alto potencial farmacológico para esta especie. Objetivo: realizar el estudio fitoquímico y evaluación de la actividad antioxidante de la especie E. litoralis. Métodos: los extractos vegetales de hojas corteza y madera se obtuvieron empleando etanol al 96 por ciento, y posteriormente fueron fraccionados usando técnicas cromatográficas como Cromatografía en Capa Delgada (CCD), Cromatografía en Columna (CC) y Cromatografía en Capa Delgada Preparativa (CCDP). Los compuestos se identificaron mediante el análisis de datos espectroscópicos con el empleo de técnicas instrumentales como: Infrarrojo (IR), Resonancia Magnética Nuclear Protónica y de Carbono trece (RMN-1H y 13C), Espectrometría de Masas (EM) y difracción de rayos x (DRX). La actividad antioxidante se evaluó a través de los métodos radical catiónico ABTS•+, radical libre DPPH• y Potencial de Actividad de Reducción Férrica (FRAP). Resultados: de esta especie se aislaron e identificaron cinco compuestos: cuatro alcaloides y un flavonoide. Los extractos en acetato de etilo de hojas y corteza mostraron una significativa actividad frente a los radicales ABTS•+ con un IC50 de 5,65 y 7,65 µg/mL respectivamente. Conclusiones: de los distintos extractos se aislaron cinco compuestos: 1-hidroxi-3-metoxi-N-metilacridona (1), maculosidina (2) maculina (3), dictamina (4) y gardenina B (5). El extracto en acetato de etilo de hojas y corteza presentaron una significativa actividad antioxidante frente al radical ABTS con un IC50 de 5,65 y 7,65 mg/L, respectivamente(AU)

Introduction: The species Esenbeckia litoralis Donn. Sm. (Rutaceae), also known as loro or loro grande, has been used in traditional medicine to treat snakebites, sore throats and lesions caused by insect bites. The compounds contained in the plant, among them lignans, terpenes, alkaloids, coumarins and polyphenols, are responsible for such a broad variety of activities, granting it great pharmacological potential. Objective: Conduct a phytochemical study and evaluation of the antioxidant activity of the species E. litoralis. Methods: Plant extracts from leaves, stem and wood were obtained using 96 percent ethanol, and then fractioned with chromatographic techniques such as thin-layer chromatography (TLC), column chromatography (CC) and preparative thin-layer chromatography (PTLC). The compounds were identified by spectroscopic data analysis using instrumental techniques such as infrared (IR) spectroscopy, proton and Carbon-13 nuclear magnetic resonance (NMR-1H and 13C), mass spectrometry (MS) and X-ray diffraction (XRD). Antioxidant activity was evaluated with the methods ABTSo+ radical cation, DPPHo free radical and ferric reducing ability power (FRAP). Results: Five compounds were isolated from the species: four alkaloids and one flavonoid. Ethyl acetate extracts from leaves and stem displayed significant activity against ABTSo+ radicals, with a CI50 of 5.65 and 7.65 µg/ml, respectively. Conclusions: Five compounds were isolated from the various extracts: 1-hydroxy-3-methoxy-N-methylacridone (1), maculosidin (2), maculin (3), dictamin (4) and gardenin B (5). The leaf and stem extract in ethyl acetate displayed significant antioxidant activity against the ABTS radical, with a CI50 of 5.65 and 7.65 mg/l, respectively(AU)

Qualitative and quantitative investigations of furocoumarin derivatives [Psoralens] of Haplophyllum tuberculatum [Rutaceae]

Haplophyllum tuberculatum is an indigenous plant widely distributed in Iraq. .Phytochemical investigation of this plant indicating that the plant is rich in alkaloids, fixed and volatik oils; but non about psoralen contents. Preliminary investigation indicated that this plant has furocoumarins in acceptable amount. Based on these results together with literature survey it was deemed desirable to carry out this phytochemical with emphasis on isolation and characterization of the furocoumarins compounds, Detailed preliminary investigation of the furocoumarins content of the dried, ground whole plant has been described and the results were discussed. Thin layer chromatography of the petroleum ether [boiling point 60-80degree C] extract demonstrated the occurance of furocoumarins compounds in the plant. The extraction procedure of the plant material, the fractionation and isolation procedures of the furocoumarins are fully described. One compound was isolated and identified as Ammoidin by TLC [compared with standard], melting point [148 C], mixed melting point [148 C] and HPLC. Spectrophotometer apparatus was used to determine exactly the amount of Ammoidin measured at 304nm at UV absorption. In Haplophyllum tuberculatum the maximum amount of Ammoidin was found sample collecting from Daquok during June.